Drimane Sesquiterpenes Isolated from the Fruiting Body of the Clavarioid Fungus Ramaria botrytoides

Four previously undescribed drimane-type sesquiterpenoids, ramaribotrytols A-D (<b>1</b>-<b>4</b>), were isolated from the fruiting bodies of the clavarioid fungus <i>Ramaria botrytoides</i>. Their structures and absolute configurations were elucidated by comprehensive 1D and 2D NMR spectroscopic analyses, high-resolution electrospray ionization mass spectrometry (HRESIMS), and electronic circular dichroism (ECD) calculations. Compounds <b>1</b>-<b>4</b> are characterized by oxygenation at C-2, a structural feature that is rare within the drimane sesquiterpene family. These findings expand the chemical diversity of secondary metabolites from the medicinal and edible fungus <i>Ramaria</i> and enrich the structural repertoire of drimane-type sesquiterpenoids derived from higher fungi.