Enhancing the efficiency of achieving optical transparency in live animals using absorbing molecules

The 5:1 tartrazine:4-AA cocktail optimally balances speed, clarity, and biosafety, advancing <i>in vivo</i> tissue-clearing technology. This strategy addresses key limitations of stand-alone dyes and expands potential applications in biomedical imaging, such as 3D tumor visualization and dynamic pathology studies. Future work should refine ratios for diverse tissues and integrate auxiliary agents (e.g., surfactants) to further enhance clearing.