ERGA-BGE reference genome of Gambusia holbrooki, a globally invasive freshwater fish

<ns3:p> The <ns3:italic>Gambusia holbrooki</ns3:italic> (eastern mosquitofish) reference genome will offer a crucial resource for understanding the evolution and adaptation of invasive freshwater fish species. The genome of <ns3:italic>G. holbrooki</ns3:italic> was assembled into two haplotypes through a phased assembly approach; however, only the primary haplotype was designated as the reference genome for annotation and downstream analyses. The entirety of the genome sequence was assembled into 24 contiguous chromosomal pseudomolecules and 1 mitochondrial genome. This chromosome-level assembly encompasses 0.67 Gb, composed of 421 contigs and 318 scaffolds, with contig and scaffold N50 values of 15.9 Mb and 29.6 Mb, respectively. </ns3:p>

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Publication Details

Journal
Open Research Europe
Published
2026-02-13
DOI
https://doi.org/10.12688/openreseurope.22693.1
Primary Topic
Genomics and Phylogenetic Studies
Type
article
Field-Weighted Citation Impact
0.00
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article

ERGA-BGE reference genome of Gambusia holbrooki, a globally invasive freshwater fish

Bonnie A. Fraser, Thomas Marcussen, Rebekah A. Oomen, Swati Sinha et al.
Open Research Europe
Genomics and Phylogenetic Studies
article

ERGA-BGE reference genome of Gambusia holbrooki, a globally invasive freshwater fish

Bonnie A. Fraser, Thomas Marcussen, Rebekah A. Oomen, Swati Sinha, Kerstin Howe, Astrid Bohne, Caroline Howard, Jonathan M.D. Wood, Clelia Gasparini, Francesco Santi, Leanne Haggerty, Chiara Bortoluzzi, Shane McCarthy, Torsten H. Struck, Fergal Martin, Mark Blaxter, Amy Denton, Rita Monteiro
article en

Abstract

<ns3:p> The <ns3:italic>Gambusia holbrooki</ns3:italic> (eastern mosquitofish) reference genome will offer a crucial resource for understanding the evolution and adaptation of invasive freshwater fish species. The genome of <ns3:italic>G. holbrooki</ns3:italic> was assembled into two haplotypes through a phased assembly approach; however, only the primary haplotype was designated as the reference genome for annotation and downstream analyses. The entirety of the genome sequence was assembled into 24 contiguous chromosomal pseudomolecules and 1 mitochondrial genome. This chromosome-level assembly encompasses 0.67 Gb, composed of 421 contigs and 318 scaffolds, with contig and scaffold N50 values of 15.9 Mb and 29.6 Mb, respectively. </ns3:p>

Open Research EuropeVol. 6
Openalex Percentile: Top 57%
Genomics and Phylogenetic Studies
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ERGA-BGE reference genome of Gambusia holbrooki, a globally invasive freshwater fish — Bonnie A. Fraser, Thomas Marcussen, et al. · Open Research Europe (2026) | TGRS Research Map | TGRS