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CoverPatch clamp recording from a right temporal cortex slice.(A) Brightfield image of patched neurons filled with biocytin.(B) Morphology reconstruction of pyramidal neuron and fast-spiking interneuron expressed in cortical layer 2/3 overlay on brightfield microscopy image.(C) Neuromorphometric reconstruction of biocytin-filled neurons with distribution of basal dendrites (red), apical dendrite (blue) and axon (yellow).(D) AP firing pattern of pyramidal neuron recorded in aCSF and their spontaneous excitatory postsynaptic currents (sEPSC) recorded at voltage clamped at -70 mV, and spontaneous inhibitory postsynaptic currents (sIPSC) recorded at -40 mV.(E) Neuronal morphology reconstruction and AP firing pattern of the pyramidal neuron recorded after 45 min action of 100 M 4-AP in aCSF, and their sEPSC recorded at -70 mV and sIPSC recorded at -40 mV.(F) Neuronal morphology reconstruction and AP firing pattern of the fast-spiking interneuron recorded after 15 min action of 100 M 4-AP in aCSF, and their epileptiform activity presenting as giant sEPSC recorded at -70 mV and this epileptiform activity recorded at -40 mV.For D-F the membrane capacitance (Cw), series resistance (Rs) and resting membrane potential (RMP) were monitored throughout the recording.(G) Zoom of events are presented as giant postsynaptic currents.For details, see Kyyriinen et al. (pp.