Identification of a novel <scp>BBWV2</scp> isolate and a sensitive and rapid <scp>RT</scp> ‐ <scp>RPA</scp> ‐ <scp>CRISPR</scp> / <scp>C</scp> as12a‐ <scp>LFD</scp> detection method development
The entire detection process can be completed within one hour without specialized equipment, requiring only visual interpretation. This RT-RPA-CRISPR/Cas12a-LFD method provides a rapid, highly sensitive, specific, and user-friendly platform for on-site detection of BBWV2, offering a practical tool for early diagnosis and disease management in agricultural settings. © 2026 Society of Chemical Industry.
Authors
- Minghao Yang (ORCID: https://orcid.org/0000-0001-5397-3576)
- Ke Han
- Bo Xie
- Meng‐ao Jia
- Chun Luo
- Yao Luo
- Tengzhi Xu
- Lei Lei
Institutions
- Guizhou Academy of Agricultural Sciences (CN)
- Tobacco Research Institute (CN)
- Guizhou Institute of Plant Protection (CN)
- Guizhou Academy of Sciences (CN)
Publication Details
- Journal
- Pest Management Science
- Published
- 2026-02-11
- DOI
- https://doi.org/10.1002/ps.70635
- Primary Topic
- Plant Virus Research Studies
- Type
- article
- Field-Weighted Citation Impact
- 0.00