Circ_0003323 binds METTL1 to mediate KLK6 m7G methylation modification and promote the progression of colorectal cancer

Colorectal cancer (CRC) is a major global health concern due to its high incidence and mortality rates. Understanding the underlying mechanisms of CRC proliferation, invasion, and metastasis is crucial for developing effective therapeutic strategies. Circular RNAs have emerged as important players in cancer development by regulating various cellular processes. N7-methylguanosine (m7G) modification has been shown to have important roles in gene expression regulation. In this study, we investigated the role of <i>hsa_circ_0003323</i> and its interaction with METTL1-mediated m7G modification in CRC development. We found that knocking down <i>hsa_circ_0003323</i> inhibited CRC cell proliferation, migration, and invasion. Further analyses revealed that <i>hsa_circ_0003323</i> interacts with METTL1 and regulates m7G modification levels in CRC cells. <i>In vivo</i> experiments showed that the combined application of <i>hsa_circ_0003323</i> knockdown and METTL1 knockdown achieved better tumor inhibitory effect. Our findings provide insights into the mechanism of CRC progression and present potential therapeutic targets for the treatment of CRC.