Single‐Nucleus Transcriptome Reveals Cellular Heterogeneity and Transcriptional Response to Heat Stress in Skeletal Muscle
We constructed the largest transcriptomic dataset, to date, for heat-exposed skeletal muscles. At tissue resolution, the response of muscles to heat stress was eliminated after a recovery period of 16 h. At single-nucleus resolution, the myofibre was the most heat-sensitive cell type, and type IIb myonuclei were the most heat-sensitive subtype of myonuclei. To our knowledge, this is the first study to reveal the molecular mechanisms underlying heat-induced muscle injury and repair at the single-cell transcriptional level.
Authors
- Li Chen
- Z. Jeffrey Chen (ORCID: https://orcid.org/0000-0001-5006-8036)
- Ziyin Han
- Li Chen (ORCID: https://orcid.org/0000-0001-5561-7612)
- Hui Li (ORCID: https://orcid.org/0000-0001-9198-3951)
- Jiaman Zhang (ORCID: https://orcid.org/0009-0002-7933-7417)
- Anan Jiang (ORCID: https://orcid.org/0000-0002-1334-6743)
- Bo Zeng
- Xiaolan Fan
- Hai Xiang
- Zhiyu Lei
- Long Jin
Institutions
- Foshan University (CN)
- Sichuan Agricultural University (CN)
- Chongqing Academy of Animal Science (CN)
- Ministry of Agriculture and Rural Affairs (CN)
Publication Details
- Journal
- Journal of Cachexia Sarcopenia and Muscle
- Published
- 2026-02-01
- DOI
- https://doi.org/10.1002/jcsm.70217
- Primary Topic
- Muscle Physiology and Disorders
- Type
- article
- Field-Weighted Citation Impact
- 0.00