Derivation and characterization of the iPSC line IITGi001-B from primary human adult dermal fibroblasts
Adult human dermal fibroblasts purchased from ATCC were reprogrammed using the Y4 combination of episomal plasmids expressing hOCT3/4, short hairpin RNA against p53, hSOX2, hKLF4, hL-MYC, and hLIN28 to generate induced pluripotent stem cells (iPSCs). A representative iPSC clone was extensively characterized to confirm the expression of pluripotency markers, normal karyotype, in vitro trilineage differentiation potential, and genetic identity using DNA fingerprinting. Further, the absence of the integration of episomal plasmids and mycoplasma contamination in the iPSC line was confirmed. The generated iPSC line can be utilized for various biological and biomedical research applications.
Authors
- Vishwas Kaveeshwar (ORCID: https://orcid.org/0000-0002-4738-7519)
- Khyati Raina (ORCID: https://orcid.org/0000-0003-4249-2720)
- Subathra Radhakrishnan (ORCID: https://orcid.org/0000-0003-3093-8342)
- Rajkumar P. Thummer (ORCID: https://orcid.org/0000-0002-4526-8364)
- Chetan Dhamne (ORCID: https://orcid.org/0000-0002-3637-2880)
- Pradeep Kumar Sundaravadivelu
- Atreyee Borthakur
- Priya Mahto
- Catherine Ann Martin
- Mohamed Rela
- Dhanlaxmi Shetty
Institutions
- Albert Einstein College of Medicine (US)
- Indian Institute of Technology Guwahati (IN)
- Homi Bhabha National Institute (IN)
- Sri Dharmasthala Manjunatheshwara College of Dental Sciences & Hospital (IN)
- Foundation for Liver Research (GB)
- Shri Dharmasthala Manjunatheshwara University (IN)
Publication Details
- Journal
- Stem Cell Research
- Published
- 2026-02-13
- DOI
- https://doi.org/10.1016/j.scr.2026.103931
- Primary Topic
- Pluripotent Stem Cells Research
- Type
- article
- Field-Weighted Citation Impact
- 0.00