Establishment of a stable monoculture system for Entodinium furca monolobum and isolation of Escherichia spp. as growth-promoting bacteria
This work not only overcomes the long-standing challenge of maintaining <i>Ent. furca monolobum</i> <i>in vitro</i> culture, but also provides a methodological framework for developing defined monoxenic systems. The stable monoculture system and identification of growth-promoting <i>Escherichia spp.</i> provide a methodological basis for mechanistic studies of protozoan-bacterial interactions in the rumen.
Authors
- Jinying He
- Yue Wang (ORCID: https://orcid.org/0000-0003-0098-5359)
- Jinmei Feng
- Wei Miao
- Bingqi Chen
- Tingyu Zheng
- Beibei Li
- Kai Liu
- Jie Xiong
- Yang Xiao
- Congjie Hua
- Dongsong Li
Institutions
- Chinese Academy of Sciences (CN)
- Jianghan University (CN)
- Institute of Hydrobiology, Biology Centre, Academy of Sciences of the Czech Republic (CZ)
Publication Details
- Journal
- Frontiers in Microbiology
- Published
- 2026-02-12
- DOI
- https://doi.org/10.3389/fmicb.2026.1741192
- Primary Topic
- Ruminant Nutrition and Digestive Physiology
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Natural Science Foundation of China
- Nanjing Agricultural University
- National Key Research and Development Program of China