IN VITRO BIOMIMETIC MICROGEL MODEL FOR LUNG CANCER

The interaction of tumour cells with the tumour microenvironment (TME) is key for survival, proliferation, migration and drug resistance. In vitro 2D models fail to reproduce TME complexity, highlighting the need for 3D biomimetic models. This work aims to develop an adaptive biomimetic culture platform for lung cancer. Alginate microspheres produced by emulsion were surface functionalized with relevant biomolecules of lung cancer stroma (collagen I, fibronectin, laminin, heparan sulphate, hyaluronic acid). A mixture of coated microspheres, called a microgel, was then used to culture H460 cancer cells for up to 7 days. Live/Dead and DNA quantification assays were performed. Within the microgel, H460 cells formed aggregates 1 (Fig. 1a). DNA quantification assays showed sustained cell growth in the microgel (Fig. 1b), while Live/Dead assay confirmed that cell viability was significantly higher in the collagen or the matrix protein mix microgel than in the control group (Fig. 1c). In summary, the 3D model provides a supportive, biomimetic environment for lung cancer culture. Future work will incorporate additional tumour niche components and evaluate response to drugs commonly used in the treatment of lung cancer, such as docetaxel or doxorubicin.