Lactic Acid Bacteria Fermentation as a Tool for Creating Texture in Plant-Based “Clean-Label” Cheeses

The growing demand for clean-label plant-based cheese alternatives underscores the need for products with desirable properties. A key technological challenge is replicating the firmness of traditional cheese without synthetic additives. This study explores lactic acid bacteria (LAB) fermentation as a natural texturizing method for clean-label plant-based cheeses. We investigated the link between LAB metabolic traits and the firmness of three substrates: cashew, soybean, and sunflower seed. A strong correlation (r ≈ −0.88) was found between final pH and firmness in cashew paste, where strains achieving a pH of ~4.0–4.5 (near the protein isoelectric point) produced the firmest gels (up to 3.35 N). Lactiplantibacillus plantarum 729/23 and Lacticaseibacillus helveticus NK-1 were most effective. Soybean paste firmness increased moderately (to 1.93 N), while sunflower seed paste showed no significant improvement (≤1.14 N) despite active acidification, indicating substrate-specific limitations. This study, supported by a comprehensive analysis of the literature on firmness measurement in plant-based cheeses, demonstrates the potential of targeted selection of natural starter cultures to create clean-label products with minimal ingredients.

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Publication Details

Journal
Fermentation
Published
2026-02-12
DOI
https://doi.org/10.3390/fermentation12020111
Primary Topic
Probiotics and Fermented Foods
Type
article
Field-Weighted Citation Impact
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article

Lactic Acid Bacteria Fermentation as a Tool for Creating Texture in Plant-Based “Clean-Label” Cheeses

Anna Shiker, I. A. Demyanenko, З. Б. Намсараев, О. С. Корнеева et al.
Fermentation
Probiotics and Fermented Foods
article

Lactic Acid Bacteria Fermentation as a Tool for Creating Texture in Plant-Based “Clean-Label” Cheeses

Anna Shiker, I. A. Demyanenko, З. Б. Намсараев, О. С. Корнеева, Egor Parkhomenko, Valeria Novikova, Alexey Sazonov, Anastasia Vostrikova
article en

Abstract

The growing demand for clean-label plant-based cheese alternatives underscores the need for products with desirable properties. A key technological challenge is replicating the firmness of traditional cheese without synthetic additives. This study explores lactic acid bacteria (LAB) fermentation as a natural texturizing method for clean-label plant-based cheeses. We investigated the link between LAB metabolic traits and the firmness of three substrates: cashew, soybean, and sunflower seed. A strong correlation (r ≈ −0.88) was found between final pH and firmness in cashew paste, where strains achieving a pH of ~4.0–4.5 (near the protein isoelectric point) produced the firmest gels (up to 3.35 N). Lactiplantibacillus plantarum 729/23 and Lacticaseibacillus helveticus NK-1 were most effective. Soybean paste firmness increased moderately (to 1.93 N), while sunflower seed paste showed no significant improvement (≤1.14 N) despite active acidification, indicating substrate-specific limitations. This study, supported by a comprehensive analysis of the literature on firmness measurement in plant-based cheeses, demonstrates the potential of targeted selection of natural starter cultures to create clean-label products with minimal ingredients.

FermentationVol. 12(2)
Kurchatov Institute (RU), Sirius University of Science and Technology (RU), Gamalei Institute of Epidemiology and Microbiology (RU), Voronezh State University of Engineering Technologies (RU)
Industry, innovation and infrastructure
Openalex Percentile: Top 57%
Probiotics and Fermented Foods
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