Ameliorative effects of gum Arabic–coated selenium nanoparticles on Cisplatin-induced cerebellar neurotoxicity in rats

Selenium nanoparticles were prepared by using Arabic gum as the stabilizer in a facile synthetic approach. The current study aims to evaluate the possible therapeutic role of alone and combined with on for protecting the cerebellum against cisplatin-induced neurotoxicity. Twenty male albino rats were randomly divided into four groups (5 male/group) as the following: Control group: The rats were injected intraperitoneally with 0.9% saline daily for 30 days. Arabic gum (AG) coated selenium nanoparticles group: The rats received Nano Selenium (0.5 mg/kg) coated with Arab Gum (7.5 mg/kg) orally for 30 days. Cisplatin group: The rats received intraperitoneally a single dose of cisplatin (5 mg/kg) Cisplatin + Arab gum coated selenium nanoparticles group: The rats received intrapritoneally a single dose of cisplatin (5 mg/kg) and nano-selenium (0.5 mg/kg) coated with Arab gum (7.5 mg/kg) orally for 30 days.Combined modality of both cis and AG.SeNPs depicted a significant increased in glutathione (GSH), Catalase (CAT) and Super oxide dismutase (SOD), content. While markedly decreased 8-hydroxy-2’-deoxyguanosine (8-OHdG), Malondialdehyde (MDA) and Hydrogen peroxide (H2O2), levels as compared to cisplatin group. Furthermore, caspase-3 and P53 levels were mitigated. Dopamine and Serotonin levels markedly increased compared to the cisplatin intoxicated group. Moreover, tumor necrosis factor-alpha (TNF-α), cytokine interleukin (IL)-6 (IL-6) and cytokine interleukin (IL)-10 (IL-10) concentrations and level were significantly decreased with treatment. Histological examinations confirmed the recuperating effects of AG.SeNPs on cerebellar tissue intoxicated with cisplatin which was indicated by intact folia, and restored Purkinje cells number and architecture. In conclusion, Selenium nanoparticles coated with Arabic gum could be used as an antioxidant, anti-apoptotic and anti-inflammatory agent to alleviate the toxicity of cisplatin on the rat cerebellum.