Multiplex PCR to Differentiate Monkeypox Virus Clades
We designed a multiplex quantitative PCR to differentiate monkeypox virus clades. For clinical samples collected in the United Kingdom and Nigeria, sensitivity was 78% (95% CI 67.67%-86.14%) and specificity 94% (95% CI 80.84%-99.30%); for samples with cycle thresholds <35, sensitivity was 98% (95% CI 91.72%-99.96%) and specificity 94% (95% CI 80.84%-99.30%).
Authors
- Christine Goffinet (ORCID: https://orcid.org/0000-0002-3959-004X)
- Susan Gould (ORCID: https://orcid.org/0000-0002-3722-867X)
- Dorcas A. Gado (ORCID: https://orcid.org/0000-0003-2130-425X)
- Collette Allen
- Adeyinka Jeremy Adedeji (ORCID: https://orcid.org/0000-0001-5736-6466)
- Adesuyi Ayodeji Omoare (ORCID: https://orcid.org/0000-0003-4065-6509)
- Nicodemus Mkpuma (ORCID: https://orcid.org/0000-0002-6337-1613)
- Jolly Amoche Adole (ORCID: https://orcid.org/0000-0001-9231-3474)
- Afolabi Akinpelu (ORCID: https://orcid.org/0000-0002-1837-6255)
- Anushri Somasundaran
- Abdulakeem Eniola Amoo
- Adeleye Adesola Semiu
- Samuel Oluwafunmbi Ifabumuyi
- Esto Bahizire
- Caitlin Greenland-Bews
- Laure Bosquillon de Jarcy
- Christopher T. Williams
- Cristina Leggio
- Thomas Edwards
- Ana Cubas-Atienzar
- Thomas E. Fletcher
- The International Severe Acute Respiratory
- Malcolm G. Semple
- Alessandra Romero-Ramirez
- Abisola Ajoke Adeyemi
- Jake Dunning
Publication Details
- Journal
- Emerging infectious diseases
- Published
- 2026-02-01
- DOI
- https://doi.org/10.3201/eid3202.250686
- Primary Topic
- Poxvirus research and outbreaks
- Type
- article
- Field-Weighted Citation Impact
- 0.00