Choice of fixative affects programmed death‐ligand 1 expression in cell blocks from pleural effusions with metastatic pulmonary adenocarcinoma
PD-L1 ICC expression in CBs depends on the fixative and antibody used. Alcohol-based fixatives, particularly with low cutoffs, risk underestimating PD-L1 positivity, and may contribute to false-negative results. ICC protocol optimization is essential before diagnostic use.
Authors
- Rita Pedersen
- Tomas Seidal
- Levent M. Akyürek (ORCID: https://orcid.org/0000-0002-1968-6458)
- Kim Hejny
- Louise Pettersson (ORCID: https://orcid.org/0000-0002-4480-4380)
- Ulrich Mager
- Ivana Kholová
- Frida Kronqvist
- Mohammed S. I. Mansour
- Anna Aronsson
Institutions
- Tampere University (FI)
- University of Eastern Finland (FI)
- Malmö University (SE)
- Lund University (SE)
- Kristianstad University (SE)
- Sahlgrenska University Hospital (SE)
- Kuopio University Hospital (FI)
- Fimlab (Finland) (FI)
- Landstinget i Kalmar län (SE)
- Hallands sjukhus Halmstad (SE)
- Halmstad University (SE)
- University of Gothenburg (SE)
Publication Details
- Journal
- Cancer Cytopathology
- Published
- 2026-02-09
- DOI
- https://doi.org/10.1002/cncy.70078
- Primary Topic
- Occupational and environmental lung diseases
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Tampereen Tuberkuloosisäätiö
- Japan Society for the Promotion of Science