PFAS contamination in Florida estuarine fishes: Levels, patterns, and estimated human and ecological health risks

Per- and polyfluoroalkyl substances (PFAS) pose a significant health threat due to their environmental persistence and toxicity. While PFAS contamination is widespread in Florida, the state currently lacks fish consumption advisories (FCAs) for these compounds, despite existing FCAs for legacy pollutants. This study quantified 40 PFAS in edible muscle tissue from 264 fish (16 species) across four estuaries along Florida's Atlantic coast to assess ecological and human health risks. The highest concentrations of total PFAS were found in Red Drum (Sciaenops ocellatus; 0.209 - 51.6 ng/g wet weight) and Spotted Seatrout (Cynoscion nebulosus; 2.01 - 24.3 ng/g ww) in the Indian River Lagoon, where up to 68% of Red Drum and 75% of Spotted Seatrout exceeded ecological quality standards, indicating potential impacts on predators. PFOS was the predominant PFAS, driving ecological and human health risks. Estimated daily intakes (EDIs) of PFOS exceeded the EPA reference dose (RfD) by up to 3 orders of magnitude, with the highest exposures concentrated in Red Drum and Spotted Seatrout from the Indian River Lagoon. These findings highlight that PFAS monitoring of tissue concentrations in fish across Florida's freshwater and marine systems would be needed for accurately quantifying exposure risks. Such efforts are fundamental to informing regulatory frameworks regarding FCAs and ensuring the long-term protection of aquatic ecosystems and human health.