Establishment of a CRISPR/Cas9 protocol by biolistic transformation of the filamentous basidiomycete Pleurotus ostreatus

Genome editing was successfully performed by introducing a CRISPR/Cas9 expression vector through biolistic transformation of Pleurotus ostreatus, an oyster mushroom. This method can be applied for genome editing in filamentous basidiomycetes without protoplasting. To our knowledge, this is the first report of genome editing with biolistic transformation in filamentous basidiomycetes.