RPLC- and HILIC-based non-targeted metabolomics workflow for blood microsamples
We developed and optimized a 15-minute HILIC-MS method that included column re-equilibration. Our experiments showed that using a 20% H<sub>2</sub>O/80% MeOH (v/v) mixture for extraction, with sample rehydration, is a good compromise for detecting many metabolite features. Our extraction and LC-MS methodology covered metabolites from many pathways, including amino acids, acylcarnitines, and bile acids.
Authors
- Michael A. Witting (ORCID: https://orcid.org/0000-0002-1462-4426)
- Pauline Couacault
Institutions
- Helmholtz Zentrum München (DE)
- Technical University of Munich (DE)
Publication Details
- Journal
- Metabolomics
- Published
- 2026-02-09
- DOI
- https://doi.org/10.1007/s11306-026-02402-y
- Primary Topic
- Metabolomics and Mass Spectrometry Studies
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- European Commission
- Aristotle University of Thessaloniki