Evaluation of Diuretic Activity of Ampelocissus Latifolia Methanolic Root Extract by In vivo and In silico Studies

Abstract The present study was designed to investigate the phytochemical constituents and evaluate the diuretic activity of the methanolic extract of Ampelocissus latifolia roots through in vivo and in silico approaches. Preliminary phytochemical analysis revealed the presence of various secondary metabolites, including flavonoids, alkaloids, tannins, glycosides, saponins, and phenolic compounds, which are known to contribute to diuretic and renal-modulating effects. The diuretic activity was assessed in experimental animal models, using standard protocols to monitor urine output, electrolyte excretion, and key indices related to renal function. The extract demonstrated a significant increase in diuresis compared to the control group, suggesting dose-dependent activity. Furthermore, in silico molecular docking studies were conducted to predict the interaction of selected phytoconstituents with diuretic targets such as the carbonic anhydrase enzyme. The docking results supported the in vivo findings, indicating strong binding affinities and potential inhibition mechanisms. The combined data from experimental and computational analyses suggest that Ampelocissus latifolia root extract exhibits promising diuretic properties and could serve as a potential source for the development of natural diuretic agents. In silico molecular docking was performed against the receptor [PDB ID : 5OW8 ] using Furosemide , Campesterol, Hexadecanoic acid , Squalene and 5 Hydroxymethylfurfural.