<i>Nodosilinea jinyiensis</i> and <i>Nodosilinea qiaokuensis</i> –two novel species of <i>Nodosilinea</i> (Nodosilineaceae, Cyanobacteria) based on a polyphasic approach

Three strains of fine filamentous cyanobacteria were isolated from freshwater lakes in Hubei province, China. A polyphasic analysis based on morphology, ecology, 16S rRNA gene phylogenies, and 16S-23S internal transcribed spacer (ITS) rRNA region secondary structures was undertaken. Based on the 16S rRNA gene phylogeny, three strains (JYH02, JYH03, and ZYHGY02) are likely new species of Nodosilinea, sharing <98.7% similarity to other species. Furthermore, the ITS rRNA region secondary structures from all three strains were distinct and unique compared with other Nodosilinea species. These results support the establishment of two new species: Nodosilinea jinyiensis sp. nov. and Nodosilinea qiaokuensis sp. nov.