Process Optimization and Functional Evaluation of <i>Polygonatum sibiricum</i> Polysaccharides Pressed Candy: A Dietary Supplement Designed for Buccal Absorption

ABSTRACT Polysaccharides are the key bioactive components of Polygonatum sibiricum , an edible plant renowned for its antioxidant, anti‐fatigue, and immunomodulatory properties. However, the extraction and preparation processes of Polygonatum sibiricum polysaccharides (PSP) and their formulation into pressed candy remain inadequately optimized. To address the challenge, this study established an efficient protocol for both PSP extraction and pressed candy preparation utilizing single‐factor analysis, orthogonal array design, and response surface methodology. The efficacy of the resulting PSP pressed candy was subsequently assessed using a series of in vitro and in vivo assays, confirming its antioxidation, antifatigue, and immunomodulation. These optimized techniques provide a robust technical framework for the industrial commercialization of PSP‐based dietary supplements. Practical Applications: This study establishes an optimized protocol for the extraction and preparation of Polygonatum sibiricum polysaccharides (PSP) to produce PSP pressed candy (PC). These refined techniques significantly enhance PSP yield and PC quality. PC exhibits robust antioxidant, anti‐fatigue, and immunomodulatory activities, as confirmed by comprehensive in vitro and in vivo evaluations. Consequently, this research provides a scalable technical framework for the industrial production of PC, positioning it as a promising candidate for functional foods and dietary supplements. These findings also expand the utilization of plant‐derived polysaccharides within the nutraceutical industry. Notably, PC is distinct from conventional solid dosage forms. It is specifically engineered for oral dissolution to facilitate buccal absorption, offering a novel strategy to bypass hepatic first‐pass metabolism and maximize bioavailability.