Cell-free DNA from cerebrospinal fluid cytology specimens as a novel liquid biopsy approach for pediatric patients with primary central nervous system tumors
This study demonstrates the feasibility of utilizing routinely discarded supernatants from CSF cytology specimens for LP-WGS and targeted NGS. Our approach optimizes the use of CSF that may be limited in pediatric patients as a source for liquid biopsy-based genomic studies. Future research will be necessary to optimize and validate the methodology to enable clinical implementation.
Authors
- Yan Chen Wongworawat (ORCID: https://orcid.org/0000-0003-0430-0265)
- Saied Mirshahidi (ORCID: https://orcid.org/0000-0003-1747-8070)
- Minwoo Song
- Pramil N. Singh (ORCID: https://orcid.org/0000-0002-9820-2887)
- Ravi I. Raghavan
- Bin Haji Ross Othman (ORCID: https://orcid.org/0000-0001-9754-399X)
- Sujata Mushrif
- Isaac Kremsky
- Adina Achiriloaie
- Udochukwu Oyoyo (ORCID: https://orcid.org/0000-0002-6284-3148)
- Brian S. Ferguson (ORCID: https://orcid.org/0000-0003-1310-9542)
- Tanya Minasian
- Cindy Fong
- Jaclyn A. Biegel
- Rajeev Nowrangi
- Edwina Choung
- Kevin Leeper
- Diem Kieu Tran
- Laura A. T. Kagami
- Muhammad Baig
- Brandon Edelbach
- Gary Yu
- Richard A. Robison
- D. Gigi Ostrow
- Nicholas Chun
Institutions
- University of Southern California (US)
- Children's Hospital of Los Angeles (US)
- Loma Linda University (US)
- Columbia University (US)
Publication Details
- Journal
- Acta Neuropathologica Communications
- Published
- 2026-02-05
- DOI
- https://doi.org/10.1186/s40478-026-02248-4
- Primary Topic
- Glioma Diagnosis and Treatment
- Type
- article
- Field-Weighted Citation Impact
- 0.00