Protein biomarker discovery in neural tissue: a comparative analysis of immunoassay and colloidal gold lateral flow assay method

The ELISA demonstrated superior sensitivity and accuracy for AChE in formalin-fixed neural tissue extracts, detecting low concentrations that the colloidal gold lateral flow assay missed. While the rapid strip test may be useful for quick yes/no identification of high-abundance biomarkers in nerve samples, it lacks the sensitivity and quantitative precision necessary for reliable neural protein diagnostics. Integration of more sensitive detection labels or reader devices would be required to bridge the performance gap between lateral flow assays and ELISA in this context.