A single microRNA miR-195 rescues the arrested B cell development induced by EBF1 deficiency

Accumulated studies have reported that hematopoietic differentiation was primarily regulated by transcription factors. Early B cell factor 1 (EBF1) is an essential transcription factor for B lymphopoiesis. Contrary to the canonical notion, we found that a single miRNA, miRNA-195 ( Mir195 ) transduction let Ebf1 -deficient hematopoietic progenitor cells (HPCs) express CD19, carry out V(D)J recombination and class switch recombination, which implied that B cell matured without EBF1. A part of the mechanism was caused by FOXO1 accumulation via inhibition of FOXO1 phosphorylation pathways in which targets of Mir195 are enriched. These results suggested that some miRNA transductions could function as alternatives to transcription factors.

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Publication Details

Journal
eLife
Published
2026-01-28
DOI
https://doi.org/10.7554/elife.101510.3
Primary Topic
FOXO transcription factor regulation
Type
article
Field-Weighted Citation Impact
0.00
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article

A single microRNA miR-195 rescues the arrested B cell development induced by EBF1 deficiency

Yuji Miyatake, Takahiro Kisikawa, Katsuto Hozumi, Ryo Yanagiya et al.
eLife
FOXO transcription factor regulation
article

A single microRNA miR-195 rescues the arrested B cell development induced by EBF1 deficiency

Yuji Miyatake, Takahiro Kisikawa, Katsuto Hozumi, Ryo Yanagiya, Kiyoshi Ando, Ryutaro Kotaki, Hiroshi Kawamoto, Reo Maruyama, Masato Ohtsuka, Takeshi Kamakura, Tomohiro Kurosaki, Chikako Shibata, Ai Kotani, Kazuaki Kameda, Ryo Koyama Nasu, Hiroyuki Hosokawa, Ken-ichi Hirano, Kazuki Okuyama
article en

Abstract

Accumulated studies have reported that hematopoietic differentiation was primarily regulated by transcription factors. Early B cell factor 1 (EBF1) is an essential transcription factor for B lymphopoiesis. Contrary to the canonical notion, we found that a single miRNA, miRNA-195 ( Mir195 ) transduction let Ebf1 -deficient hematopoietic progenitor cells (HPCs) express CD19, carry out V(D)J recombination and class switch recombination, which implied that B cell matured without EBF1. A part of the mechanism was caused by FOXO1 accumulation via inhibition of FOXO1 phosphorylation pathways in which targets of Mir195 are enriched. These results suggested that some miRNA transductions could function as alternatives to transcription factors.

eLifeVol. 13
Hiroshima University (JP), Tokai University (JP), Chiba University (JP), Tokyo University of Science (JP), Kyoto University (JP), Cancer Institute (WIA) (IN), Okayama University Hospital (JP), RIKEN Center for Integrative Medical Sciences (JP), Osaka International Cancer Institute (JP), Japanese Foundation For Cancer Research (JP), The University of Tokyo (JP), Epigenomics (Germany) (DE), The University of Osaka (JP)
Openalex Percentile: Top 66%
FOXO transcription factor regulation
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