Development of a stable replicon system of a pangolin merbecovirus for antiviral screening
• A replicon of the pangolin HKU4-related coronavirus (MjHKU4r-CoV-1) containing a nanoluciferase reporter gene was constructed. • A stable BHK21 cell line carrying the replicon has been generated. • This replicon system has been effectively employed in testing the antiviral activity of replication-targeting coronavirus drugs. • It offers a safe, efficient and convenient tool for screening antivirals against MjHKU4r-CoV-1 and other coronaviruses.
Authors
- Chunguang Wu
- Jianzhong Chen (ORCID: https://orcid.org/0000-0002-8232-7298)
- Yi-Xiang Wang (ORCID: https://orcid.org/0000-0002-5246-5497)
- Bo Zhu
Institutions
- National Medical Products Administration (CN)
- Guangzhou Experimental Station (CN)
- Henan Normal University (CN)
Publication Details
- Journal
- Virologica Sinica
- Published
- 2026-02-01
- DOI
- https://doi.org/10.1016/j.virs.2026.02.004
- Primary Topic
- SARS-CoV-2 and COVID-19 Research
- Type
- article
- Field-Weighted Citation Impact
- 0.00