Total Synthesis of Conjugation‐Ready Tetrasaccharide Repeating Units of <i>Pseudomonas Aeruginosa</i> (Lányi) O11

Herein, we report the first total synthesis of the conjugation-ready tetrasaccharide repeating units of Pseudomonas aeruginosa (Lányi) O11. The all-6-deoxy-sugars containing tetrasaccharides were synthesized using an efficient convergent methodology employing a (2 + 2) glycosylation strategy. A key to this approach is utilizing a common disaccharide donor, which streamlines the assembly of both the tetrasaccharides by minimizing protecting group manipulations and enhancing overall efficiency. The disaccharide donor was synthesized through a highly chemoselective activation of a meticulously designed reactive thioglycoside donor, allowing for precise control over stereochemistry and regioselectivity during the glycosylation.