Dataset of infrared absorption responses of leaves from five Cannabis sativa L. varieties treated with a Trichoderma spp.-based biomass suspension

This dataset provides Attenuated Total Reflectance-Fourier Transform Infrared (ATR-FTIR) spectra of <i>Cannabis sativa</i> L. leaves from five industrial hemp varieties cultivated under field conditions in Paraguay. In total, the dataset comprises 80 ATR-FTIR spectra, corresponding to five varieties, two treatment conditions, and eight biological replicates per combination. The dataset includes raw spectral profiles from placebo-treated controls and samples treated with a <i>Trichoderma</i>-based biomass suspension, organized in individual and consolidated Excel tables. These data support varietal classification, evaluation of treatment-related spectral variation, spectral library development, and applications in quality control, chemometric analysis, and preliminary regulatory screening.