Pre-clinical study on ethanolic preparations of <i>Ruta graveolens</i> and <i>Thlaspi bursa pastoris</i>

Objectives: The investigation of plant-based medicines has garnered significant attention globally due to their antioxidant properties. Various plant compounds, particularly phenols and flavonoids, contribute to this antioxidant activity. This study aimed to evaluate the antioxidant capacity and the total phenolic and flavonoid contents of ethanolic extracts of Ruta graveolens ( RG ) and Thlaspi bursa pastoris ( TBP ). Material and Methods: RG Mother tincture ( Q ) and TBP Q , along with their potencies (6C, 12C and 30C), were analysed using 2,2-Diphenyl-1-picrylhydrazyl (DPPH)-free radical scavenging and ferric ion reducing antioxidant power (FRAP) assays to determine the antioxidant power of the substances. In addition, the total phenolic content (TPC) and total flavonoid content (TFC) of both RG Q and TBP Q were assessed. Results: The findings indicated that RG Q inhibits the DPPH-free radical at 128.89 µg/mL of ascorbic acid equivalents (AAE) and the FRAP-free radical at 97.16 µg/mL of AAE. TBP Q demonstrated inhibition of the DPPH-free radical at 111.96 µg/mL of AAE and FRAP-free radical at 97.74 µg/mL of AAE. The TPC of RG Q at 100 µg/mL was 359.6 µg/mL and for TBP Q at 100 µg/mL, it was 210.8 µg/mL of gallic acid equivalents. The TFC of RG Q at 100 µg/mL was 313.37 µg/mL, while for TBP Q at 100 µg/mL, it was 89.6 µg/mL of quercetin equivalents. Conclusion: The study concluded that ethanolic extracts of RG and TBP exhibit antioxidant potential and contain substantial concentrations of phenols and flavonoids.