SIMPLseq: a high-sensitivity Plasmodium falciparum genotyping and PCR contamination tracking tool
SIMPLseq significantly extends the malaria genomic epidemiology toolkit, coupling high-sensitivity P. falciparum genotyping with PCR contamination detection in a simple laboratory protocol that uses only open-source reagents and does not require a costly pre-amplification step. Key prospective use cases for SIMPLseq include recurrent infection classification, polyclonality estimation, and genotypic infection endpoint application to intervention efficacy trials.
Authors
- Katrina Kelley
- Paulo Manrique
- Peter D. Crompton (ORCID: https://orcid.org/0000-0001-5629-0412)
- Philipp Schwabl (ORCID: https://orcid.org/0000-0002-1244-945X)
- Jorge-Eduardo Amaya-Romero
- Sean C. Murphy
- Daniel E. Neafsey
Institutions
- Broad Institute (US)
- National Institutes of Health (US)
- Friedrich-Loeffler-Institut (DE)
- Harvard University (US)
- University of Washington (US)
- National Institute of Allergy and Infectious Diseases (US)
Publication Details
- Journal
- Malaria Journal
- Published
- 2026-02-03
- DOI
- https://doi.org/10.1186/s12936-026-05796-1
- Primary Topic
- Malaria Research and Control
- Type
- article
- Field-Weighted Citation Impact
- 0.00