Reversed‐Phase High‐Performance Liquid Chromatography Method for Simultaneous Estimation of Tricyclazole and Propiconazole: Application to Quality Control of Suspoemulsion Pesticides

ABSTRACT A dependable, precise, and stability‐indicating reverse‐phase high‐performance liquid chromatographic method was developed and validated for the first simultaneous quantification of Tricyclazole and Propiconazole in a distinctive 34.2% suspension emulsion pesticide formulation. The method employed a Zodiac C 8 column (250 mm × 4.6 mm, 5 µm) maintained at 30°C, utilizing gradient elution with water (A) and a 50:50 combination of acetonitrile and methanol (B) at a flow rate of 1.2 mL/min. Detection occurred at 220 nm using a Waters Alliance 2695 system with a 2489 UV detector. Tricyclazole and Propiconazole were clearly differentiated with retention times of around 4.6 and 24.8–25.3 min, respectively. The method exhibited remarkable linearity across the 80%–120% range of the label claim, yielding correlation coefficients of 0.9948 for Tricyclazole and 0.9974 for Propiconazole. Recovery trials exhibited accuracies between 99.94% and 100.82%, with relative standard deviations under 1%. The specificity, robustness, and stability of the solution were confirmed under challenging analytical conditions. This validated method adheres to International Council for Harmonisation (ICH) Guideline Q2(R1) requirements and is suitable for regulatory and routine quality control of complex multi‐active pesticide formulations.