Suppressive Functions of Veratramine on PM2.5-Induced Oxidative Stress

<b>Background</b>: Particulate matter (PM_2.5) inhalation induces pulmonary disorders through oxidative stress. Veratramine (VRT), a steroidal alkaloid derived from Veratrum species, exhibits protective pharmacological potential. Therefore, this study aims to investigate the protective effects of VRT against PM_2.5-induced oxidative injury and the underlying molecular mechanisms. <b>Methods</b>: In vitro experiments were conducted using pulmonary artery endothelial cells (HPAECs), which were exposed to PM_2.5 (25-100 μg/mL) ± VRT (2-50 μM) or Dexamethasone (DEX; 50 μM) for 24-48 h. Measurements included 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide viability, Lactate dehydrogenase ELISA, 2',7'-dichlorodihydrofluorescein diacetate reactive oxygen species (ROS), superoxide dismutase/catalase kits, and Western blots (Bax, serum, and glucocorticoid-regulated kinase 1 (SGK1), microtubule-associated protein 1 light chain 3 (LC3), Toll-like receptor (TLR4), and mechanistic target of rapamycin (mTOR)). <b>Results</b>: PM_2.5 exposure reduced HPAEC viability in a dose- and time-dependent manner, likely due to increased lactate dehydrogenase leakage and intracellular ROS accumulation. Oxidative stress correlated with altered superoxide dismutase and catalase activities, with suppression of SGK1, a key factor in cell survival. VRT treatment enhanced cell viability, mitigated oxidative stress, and restored SGK1 expression. Moreover, VRT promoted mTOR phosphorylation and markedly suppressed PM_2.5-induced increases in TLR4, MyD88, and the autophagy markers LC3 II and Beclin 1. <b>Conclusions</b>: Collectively, these findings indicate that VRT protects against PM_2.5-induced pulmonary injury by modulating oxidative stress and the mTOR-associated autophagy pathway, highlighting its potential as a therapeutic candidate for PM_2.5-related respiratory disorders.