Ensemble Deep Learning Models for Multi-Class DNA Sequence Classification: A Comparative Study of CNN, BiLSTM, and GRU Architectures

DNA sequence classification is a fundamental problem in bioinformatics, playing an indispensable role in gene annotation and disease prediction. Whereas most deep learning models, such as CNNs, BiLSTM networks, and GRUs, have been found individually optimal, each of these methods excels in modeling a specific aspect of sequence data: local motifs, long-range dependencies, and efficient temporal modeling of the sequences. Here, we present and evaluate an ensemble model that integrates CNN, BiLSTM, and GRU architectures via a majority voting combination scheme so that their complementary strengths can be harnessed. We trained and evaluated each standalone and the integrated model on a DNA dataset comprising 4380 sequences falling under five functional categories. The ensemble model achieved a classification accuracy of 90.6% with precision, recall, and F1 score equal to 0.91, thereby outperforming the state-of-the-art techniques by large margins. Although previous studies have tried analyzing each Deep Learning method individually for DNA classification tasks, none have attempted a systematic combination of CNN, BiLSTM, and GRU based on their ability to extract features simultaneously. The current research aims at presenting a novel method that combines these architectures based on a Majority Voting strategy and proves how their combination is better at extracting local patterns and long dependency information when compared individually. In particular, the proposed ensemble model smoothed the high recall of BiLSTM with the high precision of CNN, leading to more robust and reliable classification. The experiments involved a publicly available DNA sequence data set of 4380 sequences distributed over 5 classes. Our results emphasized the prospect of hybrid ensemble deep learning as a strong approach for complex genomic data analysis, opening ways toward more accurate and interpretable bioinformatics research.