Discovery of High-Affinity Glutamine-Derived Peptides from Wheat Gliadin Targeting CaSR: a Computational Approach Integrating Deep Learning and Molecular Dynamics

Calcium-sensing receptor (CaSR)-targeting agonist peptides play a crucial role in maintaining intestinal homeostasis. In this study, we integrated deep learning, virtual screening, and molecular simulation to develop Peptide_MDI, an intelligent screening platform built on Nextflow. From 2798 candidate peptides derived from wheat gliadin, we identified RLSYQFPFYP (designated CaSR_TP_1) as the lead peptide, which binds CaSR with a dissociation constant <i>K</i>_d = 74.4 nM. Functionally, CaSR_TP_1 upregulates tight junction proteins, promotes cell proliferation, enhances antioxidant defenses, and reduces the transcriptional levels of TNF-α, IL-8, and IL-6. Mechanistically, it attenuates H₂O₂ induced oxidative stress by activating the CaSR/PLCγ1/Rac1/MAPK signaling cascade. In conclusion, the CaSR-targeting peptide RLSYQFPFYP was identified with high affinity (nanomolar range) using Peptide_MDI, establishing a scalable technological framework for discovering bioactive peptides that promote gut homeostasis and for designing next-generation smart peptide therapeutics.