Functional characterization of a dockerin-containing expansin-like protein from the anaerobic fungus Neocallimastix californiae
This study reports the production and characterization of a fungal cellulosomal expansin-like protein. The corresponding NcaEXLX1 protein and truncated variant were shown to enhance the activity of an endoglucanase, similar to observations made with non-cellulosomal expansin-like proteins. Notably, the improvement in cellulase activity upon the addition of NcaEXLX1 or NcaEXLX1tr was not correlated to extent of substrate binding.
Authors
- Taru Koitto (ORCID: https://orcid.org/0000-0001-9523-4907)
- Elizaveta Sidorova (ORCID: https://orcid.org/0000-0003-3361-2460)
- Thu V. Vuong (ORCID: https://orcid.org/0000-0002-4753-6975)
- Anna Pohto
- Merja Penttilä
- Emma R. Master
Institutions
- University of Toronto (CA)
- VTT Technical Research Centre of Finland (FI)
- Aalto University (FI)
Publication Details
- Journal
- Biotechnology for Biofuels and Bioproducts
- Published
- 2026-02-03
- DOI
- https://doi.org/10.1186/s13068-026-02744-8
- Primary Topic
- Biofuel production and bioconversion
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Concordia University
- Biocenter Finland
- Biocenter, University of Oulu