Strawberry <i>Fragaria x ananassa</i> Duchesne (cv. Romina) methanolic extract attenuates hydrogen peroxide induced DNA damage in human peripheral blood mononuclear cells <i>in vitro</i>

Among berry fruits, strawberries are known for a wide array of beneficial biological activities, including antioxidant, antihypertensive, antibacterial, anti-proliferative, anti-inflammatory, anticancer, and anti-metastatic effects. The cultivar Fragaria × ananassa Duchesne (Romina) is particularly rich in bioactive constituents that may contribute to limiting DNA damage triggered by reactive oxygen species. In this study, we examined whether the methanolic extract of Romina strawberries (RE) exerted any genotoxic effect on human peripheral blood mononuclear cells (PBMCs) across concentrations ranging from 25 to 200 μg/mL using the comet assay. No evidence of genotoxicity was detected. Subsequently, the capacity of RE to counteract oxidative DNA damage in two experimental models was assesed. When PBMCs were pretreated with RE prior to hydrogen peroxide exposure, a slight reduction in DNA damage was observed, with only the 50 μg/mL concentration producing a statistically significant protective effect. To evaluate the extract's interventional capacity, cells were treated with RE following exposure to H 2 O 2 . In this post-treatment design, RE demonstrated a clearly stronger antigenotoxic response, reducing DNA damage at all tested concentrations, with the most pronounced effects at 100, 150, and 200 μg/mL. Overall, the methanolic extract of the Romina cultivar showed notable protective activity against H 2 O 2 -induced oxidative DNA damage in vitro .