Active single-pixel microscopy for quantitative phase imaging

In this work, a motionless method to achieve quantitative phase imaging in single-pixel microscopy based on the transport of intensity equation is presented. In this approach, a digital micromirror device is used to generate wide-field structured illumination over the sample. The light resulted by the interaction between the sequence of light patterns and the sample is collected using a bucket detector. The integration of a focus tunable lens allows the motionless acquisition of multiple intensity images required for applying the transport of intensity equation. Quantitative phase retrieval in active single-pixel microscopy is demonstrated by imaging calibrated pure-phase test targets.

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Publication Details

Journal
Springer Link (Chiba Institute of Technology)
Published
2025-09-22
DOI
https://doi.org/10.1051/epjconf/202533503006/pdf
Primary Topic
Digital Holography and Microscopy
Type
article
Field-Weighted Citation Impact
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article

Active single-pixel microscopy for quantitative phase imaging

Heberley Tobòn-Maya, Samuel I. Zapata-Valencia, Jesus Lancis, Enrique Tajahuerce
Springer Link (Chiba Institute of Technology)
Digital Holography and Microscopy
article

Active single-pixel microscopy for quantitative phase imaging

Heberley Tobòn-Maya, Samuel I. Zapata-Valencia, Jesus Lancis, Enrique Tajahuerce
article en

Abstract

In this work, a motionless method to achieve quantitative phase imaging in single-pixel microscopy based on the transport of intensity equation is presented. In this approach, a digital micromirror device is used to generate wide-field structured illumination over the sample. The light resulted by the interaction between the sequence of light patterns and the sample is collected using a bucket detector. The integration of a focus tunable lens allows the motionless acquisition of multiple intensity images required for applying the transport of intensity equation. Quantitative phase retrieval in active single-pixel microscopy is demonstrated by imaging calibrated pure-phase test targets.

Springer Link (Chiba Institute of Technology)
Openalex Percentile: Top 28%
Digital Holography and Microscopy
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