Establishing a Defined Gut Bacterial Population in Adult Mosquitoes

Source: Liu, X., et al. A Bacterial Oral Feeding Assay with Antibiotic-Treated Mosquitoes. J. Vis. Exp. (2020)This video demonstrates the depletion of native gut microbiota in mosquitoes using an antibiotic-containing sucrose solution. This is followed by controlled recolonization with a bacterial suspension delivered through a membrane feeding system to establish a defined bacterial population in the mosquito gut.