Optical component integration in a microscope slide: A novel approach for super-resolution imaging

Structured illumination microscopy (SIM) is a powerful super-resolution technique that relies on patterned light to surpass the diffraction limit. However, conventional SIM setups require complex optical configurations that limit their adaptability and usability. In this work, we present an integrated optical chip fabricated using femtosecond laser micromachining, designed to simplify the generation of structured light. This chip, coupled with an optical fiber, enables direct generation of structured illumination on its surface, where the biological samples can be positioned for imaging. Our approach eliminates bulky optics, enhances system stability, and offers a compact and scalable solution for super-resolution microscopy. We present the design, the fabrication process, and the first validations of this system for advanced biological imaging.