Contemporary Advances and Future Perspectives in Rosaceae Plant Regeneration

Members of the Rosaceae family possess substantial economic and ornamental value, making their effective propagation and genetic improvement critical. Plant regeneration represents a foundational technology for efficient breeding, genetic transformation, functional genomics, molecular breeding, germplasm conservation, and large-scale commercial propagation. The regenerative capacity of explants in many Rosaceae taxa remains limited, despite significant progress. This review systematically synthesized conventional and emerging plant regeneration strategies and critically examined the principal biological and technical constraints affecting regenerative efficiency. A comprehensive comparison was first made among the various genera of the Rosaceae family regarding regeneration processes, environmental conditions, PGRs, exogenous additives, basal media, common obstacles and regeneration suggestions. The application of molecular biotechnology approaches in elucidating the mechanisms underlying regeneration and in enhancing regeneration capacity is also evaluated. Finally, this review assesses the future potential of these advanced technologies for improving regeneration systems in Rosaceae plants, providing a comprehensive reference framework for both academic research and industrial applications.