The Effect of Different Types of Commercial Diluents on the Semen Quality of

Polled Bali cattle are a variant of Bali cattle characterized by the natural absence of horn growth. In artificial insemination programs, Andromed® is a commonly used commercial semen diluent; however, limited studies have evaluated its performance compared to other diluents containing lecithin-based components. This study aimed to assess the semen quality of polled Bali bull using three commercial diluents: Andromed®, Steridyl®, and Bovifree®. The research was conducted at the UPT-PIBPS, Livestock and Animal Health Service of South Sulawesi Province, and the In Vitro Embryo Production Laboratory, Hasanuddin University. Semen was collected from two polled Bali bulls aged and 7 years. A completely randomized design (CRD) was employed with three treatment groups and seven replications. Semen quality was evaluated based on progressive motility, viability, abnormalities, and Intact Plasma Membrane (IPM). Data were analyzed using analysis of variance (ANOVA) followed by Duncan’s multiple range test. The results indicated that Andromed® significantly improved sperm motility (P<0.01) compared to Steridyl® and Bovifree®. Viability did not differ significantly among the diluents (P>0.05). Sperm abnormalities were significantly higher in the Steridyl® group (P<0.01), while Andromed® showed significantly better intact plasma membrane than Steridyl® (P<0.05), but was not substantially different from Bovifree® (P>0.05). In conclusion, Andromed® demonstrated better performance in most semen quality parameters compared to Steridyl® and Bovifree® for polled Bali bulls semen cryopreservation.