Normal table of post-embryonic larval development for the California newt, <i>Taricha torosa</i>

We present here a normal table for post-embryonic development in the California newt (<i>Taricha torosa</i>), part of a genus of newts frequently studied for their toxicity and role within a predator-prey relationship. We generated the table by observing larvae collected as eggs in the wild and hatched and reared in the lab through metamorphosis. Building upon an established table consisting of 40 embryonic stages of development, our table consists of 13 stages based on discrete anatomical changes, primarily in limb development, and concludes at Stages 12-13 when the larvae undergo metamorphosis. We also describe more gradual phenotypic changes and their correlation to discrete stages in the developmental timeline. Finally, we illustrate the variability of the timing for reaching these stages in a controlled lab environment, demonstrating that time from hatching is not a reliable metric for standardizing results for diverse studies involving developing larvae. This staging table and accompanying observations will facilitate cross-study integration of research with larval <i>T. torosa</i>.

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Publication Details

Journal
bioRxiv (Cold Spring Harbor Laboratory)
Published
2026-02-02
DOI
https://doi.org/10.64898/2026.01.30.702904
Primary Topic
Environmental Toxicology and Ecotoxicology
Type
article
Field-Weighted Citation Impact
0.00

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article

Normal table of post-embryonic larval development for the California newt, Taricha torosa

Elizabeth Heath-Heckman, Gary M. Bucciarelli, Samantha M Westcott, Heather L Eisthen
bioRxiv (Cold Spring Harbor Laboratory)
Environmental Toxicology and Ecotoxicology
article

Normal table of post-embryonic larval development for the California newt, Taricha torosa

Elizabeth Heath-Heckman, Gary M. Bucciarelli, Samantha M Westcott, Heather L Eisthen
article en

Abstract

We present here a normal table for post-embryonic development in the California newt (<i>Taricha torosa</i>), part of a genus of newts frequently studied for their toxicity and role within a predator-prey relationship. We generated the table by observing larvae collected as eggs in the wild and hatched and reared in the lab through metamorphosis. Building upon an established table consisting of 40 embryonic stages of development, our table consists of 13 stages based on discrete anatomical changes, primarily in limb development, and concludes at Stages 12-13 when the larvae undergo metamorphosis. We also describe more gradual phenotypic changes and their correlation to discrete stages in the developmental timeline. Finally, we illustrate the variability of the timing for reaching these stages in a controlled lab environment, demonstrating that time from hatching is not a reliable metric for standardizing results for diverse studies involving developing larvae. This staging table and accompanying observations will facilitate cross-study integration of research with larval <i>T. torosa</i>.

bioRxiv (Cold Spring Harbor Laboratory)
California Department of Fish and Wildlife (US), Michigan State University (US)
Michigan State University, National Institutes of Health, College of Engineering, Michigan State University
Openalex Percentile: Top 70%
Environmental Toxicology and Ecotoxicology
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