Isolation of Bacteria from the Mosquito Midgut

Source: Liu, X., et al. A Bacterial Oral Feeding Assay with Antibiotic-Treated Mosquitoes. J. Vis. Exp. (2020)This video demonstrates the dissection of the mosquito midgut to isolate gut bacteria. The midgut is extracted from the digestive tract, homogenized to release the bacteria, and plated on nutrient agar to culture bacterial colonies.

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Publication Details

Journal
Journal of Visualized Experiments
Published
2026-02-02
DOI
https://doi.org/10.3791/24342
Primary Topic
Insect symbiosis and bacterial influences
Type
article
Field-Weighted Citation Impact
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article

Isolation of Bacteria from the Mosquito Midgut

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Journal of Visualized Experiments
Insect symbiosis and bacterial influences
article

Isolation of Bacteria from the Mosquito Midgut

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article en

Abstract

Source: Liu, X., et al. A Bacterial Oral Feeding Assay with Antibiotic-Treated Mosquitoes. J. Vis. Exp. (2020)This video demonstrates the dissection of the mosquito midgut to isolate gut bacteria. The midgut is extracted from the digestive tract, homogenized to release the bacteria, and plated on nutrient agar to culture bacterial colonies.

Journal of Visualized Experiments
Openalex Percentile: Top 72%
Insect symbiosis and bacterial influences
DOISourceOpenAlex
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