Eight new α-pyrone and γ-butenolide derivatives from the plant endophytic fungus Diaporthe sp. CCY4
Five new α-pyrones, diaporpyrones G-K (1-5) and three new γ-butenolide derivatives, porbutenolides A-C (6-8), along with seven known compounds (9-15), were isolated from the culture extract of the endophytic fungus Diaporthe sp. CCY4. Their structures were elucidated by comprehensive spectroscopic analysis, including 1D/2D NMR and HRESIMS data. The absolute configurations of 7 and 8 were assigned using electronic circular dichroism (ECD) calculations. All compounds were evaluated for inhibitory activity against ubiquitin-specific peptidase 4 (USP4). Compounds 2, 5, 9, and 13 exhibited significant anti-ubiquitination effects at 40 μM, with 13 showing the most potent inhibition (IC<sub>50</sub> = 20.85 μM).
Authors
- Jie-Chun Zeng
- Wei‐Guang Wang (ORCID: https://orcid.org/0000-0003-3395-767X)
- Xuping Zhang (ORCID: https://orcid.org/0000-0001-9805-2316)
- Lu Gao
- Qian-Qian Yin
Institutions
- Minzu University of China (CN)
- Yunnan University (CN)
- State Ethnic Affairs Commission (CN)
Publication Details
- Journal
- Natural Products and Bioprospecting
- Published
- 2026-02-03
- DOI
- https://doi.org/10.1007/s13659-025-00580-1
- Primary Topic
- Microbial Natural Products and Biosynthesis
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Natural Science Foundation of China
- Natural Science Foundation of Yunnan Province
- Yunnan Provincial Department of Education
- Yunnan Provincial Department of Education Science Research Fund Project