Effect of Chitosan Oligosaccharide (COS) Supplementation on TLR4 Gene Expression, Blood Profil, and Carcass Weight in Broiler Chicken

This study was carried out to evaluate Chitosan Oligoshaccarida (COS) supplementation on TLR4 gene expression, blood profile, and carcass weight in broiler chicken. A total of 100 Lohmann broiler Day Old Chick (DOC) with an initial average body weight of 46 ± 0.7 g were randomly allotted into 2 group treatments with 5 replications (10 unsexed DOC in each pen) for 35 days. Dietary treatments were Basal Diet (BD) without supplement (P0) and BD + COS 100 mg kg-1 (P1). The results showed that treatments with COS 100 mg kg-1 decrease TLR4 mRNA expression level compared to control, but were not differ on blood profile and the ratio between heterophils and lymphocytes which ranged from 0.58-0.87 indicated that chickens experienced moderate to high stress. COS supplementation had the significant differences in carcass weight, where chickens given COS supplementation had a higher carcass weight than the control. In conclusion COS supplementation in broiler feed can significantly increase carcass weight and improve the health status of chickens as seen from blood profile and TLR4 gene expression.