Effect of External Antioxidant Supplementation on 8-Ohdg Reduction, The Increase of Testosterone, Sod2 and Spermatozoa Quality

Exposure to pesticides can negatively impact sperm quality. To enhance spermatozoa quality, non-hormonal treatments are employed as a potential intervention. This research aimed to evaluate the effect of external antioxidant supplementation on 8-OHdG reduction, testosterone and SOD2 levels, and sperm quality improvement among infertile farmers exposed to pesticides. Over a span of 12 weeks, participants were given daily doses of 40 mg of zinc, 400 mg of vitamin E, 500 mg of vitamin C, and 250 mg of glutathione. Sperm quality, 8-OHdG, SOD2, and testosterone levels were assessed before and after the intervention. The results showed a significant increase in spermatozoa concentration after supplementation (p = 0.02) and between groups (p = 0.05). However, no significant differences were observed in 8-OHdG reduction, testosterone, SOD2 levels, morphology, or motility parameters. These findings suggest that antioxidant supplementation may effectively improve sperm concentration in men with pesticide-related infertility.