Autotrophic methylotrophy with no methanol dehydrogenase (MDH) in a strain of fluorescent <i>Pseudomonas</i>

To the author's knowledge, all wild-type methylotrophic <i>Pseudomonadota</i> (<i>i.e.</i>, "Gram-negative bacteria") so far described employ a specific dehydrogenase distinctively adapted to using methanol as a substrate (MxaFI, XoxFI, or Mdh2). The methylotrophic <i>Pseudomonas</i> strains described here lack MDH and employ generalist ADHs, thus demoting methanol dehydrogenase (MDH) from the position of a critical enzyme for methanol utilization and expanding the range of enzymes (and genes) that enable methylotrophy in nature. The second remarkable result of this work is the discovery of the utilization of the CBB cycle by a <i>Pseudomonas</i> strain during methylotrophic growth, an absolute novelty for this very relevant bacterial genus.