Isolation of Mouse Bladder Epithelial Cells Containing Intracellular Bacterial Communities

Source: Yang, E., et al. Isolation of Single Intracellular Bacterial Communities Generated from a Murine Model of Urinary Tract Infection for Downstream Single-cell Analysis. J. Vis. Exp. (2019)This video demonstrates the manual isolation of single epithelial cells containing intracellular bacterial communities from infected mouse bladder using mouth micropipetting, enabling downstream single-cell analysis of viable host-associated E. coli populations in urinary tract infection models.