Fully quantitative transmission Optical Coherence Tomography for analysis of multiple-scattering samples

We present a fully quantitative full-field optical coherence tomography realized in transmission. This method allows, for the first time, a direct and fast retrieval of the complex amplitude of light that propagated through the sample of interest. We show that with an appropriate spectral range of the swept-source laser, this method separates weakly-scattered photons from the multiply-scattered ones, thus allowing measurement of thick and scattering samples, like tissue slices or organoids. With a relatively easy optical setup and straightforward numerical processing, this method is superior to classical quantitative phase imaging methods like digital holographic microscopy.