Nanozyme-Based Colorimetric Assay on a Magnetic Microfluidic Platform for Integrated Detection of TTX
Tetrodotoxin (TTX) is a potent marine neurotoxin, necessitating sensitive and user-friendly on-site assays. To address long workflows of traditional immunoassays and limited signal amplification in colorimetric microfluidics, we developed a nanozyme-catalyzed colorimetric magnetic microfluidic immunosensor (Nano-CMI). This platform combines an aptamer-antibody sandwich capture format with catalytic amplification via AuNR@Pt@m-SiO<sub>2</sub> (APMS) nanozymes on a magnetically actuated microfluidic chip. Magnetic actuation simplifies sample handling and washing, while APMS catalysis enhances sensitivity and visual readout. The Nano-CMI has been used for the detection of TTX samples ranging from 0.2 to 20 ng/mL with a detection limit of 0.2 ng/mL in 10 min, following the linear equation: y = -31.14ln x + 110.15, and the entire "capture-reaction-detection" workflow can be completed within 1 h. With rapid response, minimal hands-on time, and robust performance, this platform offers a practical, high-sensitivity solution for on-site TTX screening in food safety and customs inspection.
Authors
- Chenqi Zhang (ORCID: https://orcid.org/0009-0007-9996-0536)
- Chang Chen
- Shuo Wu
- Jianlong Zhao
- Fangzhou Zhang
- Bo Liu
Institutions
- Shanghai University (CN)
- University of Shanghai for Science and Technology (CN)
- Chinese Academy of Sciences (CN)
- Ruijin Hospital (CN)
- State Key Laboratory of Transducer Technology (CN)
- Shanghai Institute of Microsystem and Information Technology (CN)
Publication Details
- Journal
- Biosensors
- Published
- 2026-02-01
- DOI
- https://doi.org/10.3390/bios16020089
- Primary Topic
- Marine Toxins and Detection Methods
- Type
- article
- Field-Weighted Citation Impact
- 0.00