Efecto de la suplementación con Trolox y resveratrol durante la refrigeración de semen porcino

This research aimed to evaluate the possibility of improving the boar semen quality already refrigerated in commercial diluent by adding Trolox and/or resveratrol during the refrigeration process. Pools of refrigerated semen from boars of proven fertility were added with a) 200 µM Trolox, b) 50 µM resveratrol, c) 200 µM Trolox + 50 µM resveratrol, or d) no antioxidant supplementation (negative control) and conserved at 17 °C for 7 days. On days 1, 3 and 7 of refrigeration the following sperm parameters were evaluated: Motility, functions of the plasma membrane, pre-capacitated spermatozoa, viability and acrosome integrity, and mitochondrial membrane potential. Additionally, mitochondrial superoxide anion production was evaluated by flow cytometry. In vitro fertilization was performed using semen from day 3 of refrigeration. Data were analyzed using ANOVA (P<0.05). Evaluated parameters significantly decreased over refrigeration time (P<0.05), except the percentages of pre-capacitated spermatozoa. Samples supplemented only with Trolox maintained values similar to the control group, except for higher sperm motility on day 3 of preservation (P<0.05), whereas the addition of resveratrol caused a decrease in the studied parameters (P<0.05); the combination of both antioxidants showed intermediate values between each individual antioxidant treatment. No significant variations in mitochondrial superoxide anion production and cleavage rates were detected with each treatment with respect to the control. In conclusion, supplementing boar semen with Trolox and/or resveratrol does not cause a significant improvement in semen quality, once the refrigeration process has started, except for a higher motility with Trolox at day 3 of refrigeration, nor does it alter the mitochondrial production of reactive oxygen species and the fecundity capacity of semen in vitro.