Microbiota bacteriana do leite bovino cru armazenado em um centro de coleta

Introduction: Knowing the bacterial microbiota present in raw bovine milk is important to determine its safety and possible uses in the production of dairy products. Objective: Identify the biodiversity of the bacterial microbiota of raw bovine milk stored in a collection center in Ecuador. Methods: Illumina MiSeq next-generation sequencing (NGS) of the V4 hypervariable region of the 16S rRNA gene was used to identify the bacterial taxonomic composition of the analyzed milk. The study was carried out during the winter and summer months in the Province of Tungurahua, Ecuador and the samples were collected in a collection center. The comparative analysis of the sequences obtained was carried out using the ClustalW and BLAST programs, obtaining the phylotypes assigned at the family, genus, and species level. Results: The data obtained indicate a predominance of Gram positive bacteria (Terrabacteria) belonging to the group of lactic acid bacteria (Firmicutes), highlighting the majority presence, both in winter and summer, of the species Lactococcus raffinolactis (42.3% and 22 .81%) and Lactococcus lactis (37.4% and 52.25%). Biodiversity and species richness were greater in the winter season, although the number of bacteria was greater in the summer season. Pathogenic microorganisms were also identified, although in low percentages, of the genera Acinetobacter, Aeromonas, Klebsiella, Pseudomonas, Staphylococcus and Streptococcus. Conclusions: The research findings can help formulate strategies for the production of good quality dairy products and establish designations of origin that enhance their commercialization.